Permeability Of Hydrophilic Permeability of Hydrophilic Supervisors: Vladan Milovic Professor Per Artursson thickset Investigations of the integrity and station characteristics of 2/4/A1 cells have been through in this report. The cell line was isolated from rat fetal intestinal epithelial cells and transfected with thermolabile SV40 large T antigen. These cells proliferated at 33 °C, but eliminated the antigen and ceased proliferating at a non-permissive temperature (39°C). At 39°C 2/4/A1 cells started to differentiate but simultaneously the cells also underwent massive cell death.
When cultured at 37°C these cells formed confluent and tight monolayers that seemed to have paracellular transport characteristics similar to that of the human intestine. Transmission electron microscopy confirmed the phylogeny of multilayers at 33°C, monolayers at 37°C and defects in the cell layer due to apoptosis at 39°C. Different immunostainings of ZO-1, E-cadheri...If you want to get a full essay, localise it on our website:
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