Biotechnological Processes

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Biotechnological Processes

Mo El-Aloul

SBI 4U

Mr. Daviult

May 12, 2005

Biotechnological Processes: Somatropin Harvesting

        The use of living organisms, or organic substances, to initiate new products or processes is not something new. Genetic manipulation has been performed for thousands of years. This is seen in the modern dog. They are countless variation of the canine breed. public have bread dogs to fascinateher to obtain desirable properties, much(prenominal) as a strong bite, or a veritable colour. It is not until recently that the genetic manipulation is being performed at molecular level. Genetic manipulation may seem complex, solely are essentially simple manipulation of the genetic economy casing profound results.

        Take for example somatropin, a genetically engineered version of the human growth internal secretion somatotropin (Di Giuseppe, et al, 293) . out front the production somatropin, somatotropin was extracted from the pituitary of cadavers, a procedure that risks the gap of infectious diseases, such as Creutzfeldt-Jakob disease (Di Giuseppe, et al, 293). Somatropin is used to lot growth deficiencies caused by genetic mutations or diseases (Di Giuseppe, et al, 293), and is used illegally by athletes to gain muscle.

        The procedure used to harvest somatropin is quite simple in overall context.



All that is needed is the primary winding human growth hormone genes, a colloidal gel dielectrolysis a biological sieve of sorts, a plasmid, and E. coli bacteria (Di Giuseppe, et al, 294). The gel electrophoresis is used to extract the purified human growth hormone gene. After the gene is extracted, it is ligated using desoxyribonucleic acid ligase. An enzyme the joins complementary strands by forming the phosphodiester bonds of the DNA backbone (Di Giuseppe, et al, 289). The gene is ligated to a plasmid, circular DNA that can be used as a sender to deliver the growth gene inside the E. coli. The E.

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